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En protokoll for Decellularizing musen Cochleae indre øret Tissue engineering
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Journal JoVE Bio-ingénierie
A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering

En protokoll for Decellularizing musen Cochleae indre øret Tissue engineering

DOI:
10.3791/56523-v

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09:53 min

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January 01, 2018

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, , , ,
1Department of Otolaryngology,University of Kansas Medical Center, 2Stephenson School of Biomedical Engineering,University of Oklahoma, 3Department of Plastic Surgery,University of Kansas Medical Center

Chapitres

  • 00:05Titre
  • 01:05Temporal Bone Harvest and Cochlea Isolation
  • 03:07Cochlear Processing
  • 05:11Infusion of hWJCs into Decellularized Cochleae
  • 06:58Results: Cell Culture on Decellularized, Decalcified Mouse Cochleae
  • 08:18Conclusion

Summary

Traduction automatique

Traduction automatique

Målet med denne protokollen er å demonstrere en effektiv metode for å decellularize og avkalke mus cochleae for utnyttelse som stillaser for tissue engineering programmer.

Tags

DecellularizationDecalcificationMouse CochleaExtracellular MatrixStem Cell DifferentiationSensory EpitheliumTemporal BoneBony LabyrinthStapesOval WindowRound WindowPerilymph1% SDS SolutionPBSAntibiotic-antimycoticPenicillin-streptomycin

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