Journal
/
Immunologie et infection
/
Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

DOI:
10.3791/56605-v

•

07:50 min

•

December 08, 2017

•

, , ,
1Department of Cancer Biology and Genetics,The Ohio State University College of Medicine

Chapitres

  • 00:05Titre
  • 00:35Nickel Affinity Chromatography
  • 03:30Heparin Affinity Chromatography
  • 04:28Nuclease Assay
  • 06:46Results: Nuclease Assay of Heparin Sepharose Fractions
  • 07:19Conclusion

Summary

Traduction automatique

Traduction automatique

Recombinant prototype foamy virus integrase protein is often contaminated with a bacterial nuclease during purification. This method identifies nuclease contamination and removes it from the final preparation of the enzyme.

Tags

Nuclease ContaminationProtein PurificationRecombinant Prototype Foamy Virus IntegraseE. ColiSonicationUltracentrifugationFPLCNickel-charged ResinSDS-PAGEUV Absorbance

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/56605?article_type=v

Generation of Recombinant Influenza Virus from Plasmid DNA
check_url/fr/56605?article_type=v

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection
check_url/fr/56605?article_type=v

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
check_url/fr/56605?article_type=v

High-throughput Detection Method for Influenza Virus
check_url/fr/56605?article_type=v

Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells
check_url/fr/56605?article_type=v

In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus
check_url/fr/56605?article_type=v

Rescue of Recombinant Newcastle Disease Virus from cDNA
check_url/fr/56605?article_type=v

Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
check_url/fr/56605?article_type=v

Assembly and Purification of Prototype Foamy Virus Intasomes
check_url/fr/56605?article_type=v

Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples

Read Article