Journal
/
Biologie
/
Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder (ALLIGATOR)
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder (ALLIGATOR)

Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder (ALLIGATOR)

DOI:
10.3791/56623-v

•

10:33 min

•

December 13, 2017

•

, ,
1MRC Laboratory of Molecular Biology

Chapitres

  • 00:05Titre
  • 00:51Cell Seeding and 3-(N-Morpholino)propanesulfonic Acid (MOPS)-buffered High Glucose Medium Preparation
  • 02:52Bioluminescent Imaging of Static Tissue Cultures
  • 06:31Bioluminescent Imaging of Perfused Tissue Cultures
  • 09:19Results: Bioluminescent Imaging of Mammalian Cells Using an ALLIGATOR
  • 10:11Conclusion

Summary

Traduction automatique

Traduction automatique

Genetically encoded luciferase is a popular non-invasive reporter of gene expression. Use of an automated longitudinal luciferase imaging gas- and temperature-optimized recorder (ALLIGATOR) enables longitudinal recording from bioluminescent cells under a wide range of conditions. Here we show how ALLIGATOR may be used in the context of circadian rhythms research.

Tags

Bioluminescent ReportersLongitudinal Luciferase ImagingALLIGATORCircadian RhythmCell CultureTemperature SynchronizationDMEM MediumPH Optimization

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/56623?article_type=v

In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum
check_url/fr/56623?article_type=v

Development of automated imaging and analysis for zebrafish chemical screens.
check_url/fr/56623?article_type=v

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
check_url/fr/56623?article_type=v

LeafJ: An ImageJ Plugin for Semi-automated Leaf Shape Measurement
check_url/fr/56623?article_type=v

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish
check_url/fr/56623?article_type=v

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
check_url/fr/56623?article_type=v

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)
check_url/fr/56623?article_type=v

An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production
check_url/fr/56623?article_type=v

Measuring the Confluence of iPSCs Using an Automated Imaging System
check_url/fr/56623?article_type=v

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

Read Article