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Isolation and Cultivation of Adult Rat Cardiomyocytes
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Médecine
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Journal JoVE Médecine
Isolation and Cultivation of Adult Rat Cardiomyocytes

Isolation and Cultivation of Adult Rat Cardiomyocytes

DOI:
10.3791/56634-v

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11:06 min

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October 19, 2017

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, ,
1Institute of Physiology,Justus Liebig University Giessen

Chapitres

  • 00:05Titre
  • 01:15Preparation of Langendorff Perfusion System
  • 03:09Extraction and Isolation of the Heart
  • 08:21Results: The Appearance and Behavior of Adult Rat Ventricular Cardiomyocytes (ARVC) during Six Days of Cultivation
  • 10:02Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a protocol for the isolation and cultivation of adult rat ventricular cardiomyocytes (ARVC). Isolated ARVC can be used for short and long-term cultivation. The isolation and cultivation of ARVC can play a key role in developing new treatment regimens for cardiac diseases.

Tags

Keywords: Adult Rat CardiomyocytesCardiac BiologyCardiac PathophysiologyCardiac DedifferentiationCardiac HypertensionHeart FailureLangendorff Perfusion SystemCollagenasePowell MediumWistar Rats

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