/
/
Methodology for Biomimetic Chemical Neuromodulation of Rat Retinas with the Neurotransmitter Glutamate In Vitro
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:16Wholemount Retinal Preparation
- 03:12MEA and Data Acquisition Setup
- 05:23Glutamate Stimulation Preparation
- 08:38Retinal Recording and Stimulus Program
- 10:30Results: Electrode Recordings of Neuronal Activity During Visual, Spontaneous, and Glutamate Injection Recordings
- 12:06Conclusion
This protocol describes a novel method for investigating a form of chemical neurostimulation of wholemount rat retinas in vitro with the neurotransmitter glutamate. Chemical neurostimulation is a promising alternative to the conventional electrical neurostimulation of retinal neurons for treating irreversible blindness caused by photoreceptor degenerative diseases.
