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Posterior Semicircular Canal Approach for Inner Ear Gene Delivery in Neonatal Mouse
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Journal JoVE Médecine
Posterior Semicircular Canal Approach for Inner Ear Gene Delivery in Neonatal Mouse

Posterior Semicircular Canal Approach for Inner Ear Gene Delivery in Neonatal Mouse

DOI:
10.3791/56648-v

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03:52 min

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March 02, 2018

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,
1National Institute on Deafness and Other Communication Disorders,National Institutes of Health, 2Department of Otolaryngology-Head & Neck Surgery,Johns Hopkins School of Medicine

Chapitres

  • 00:05Titre
  • 00:32Procedure Setup
  • 01:08Surgical Approach
  • 02:35Results: Transduction of Utricular and Cochlear Hair Cells
  • 03:22Conclusion

Summary

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Traduction automatique

In this study, we describe the posterior semicircular canal approach as a reliable method for inner ear gene delivery in neonatal mice. We show that gene delivery through the posterior semicircular canal is able to perfuse the entire inner ear.

Tags

Posterior Semicircular CanalInner Ear Gene DeliveryNeonatal MouseAAV8-whirlinGene TherapyHair Cell TransductionStereocilia ElongationSurgical DissectionMicro-injection

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