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Pre-Conditioning the Airways of Mice with Bleomycin Increases the Efficiency of Orthotopic Lung Cancer Cell Engraftment
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Médecine
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Journal JoVE Médecine
Pre-Conditioning the Airways of Mice with Bleomycin Increases the Efficiency of Orthotopic Lung Cancer Cell Engraftment

Pre-Conditioning the Airways of Mice with Bleomycin Increases the Efficiency of Orthotopic Lung Cancer Cell Engraftment

DOI:
10.3791/56650-v

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09:02 min

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June 28, 2018

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, ,
1Department of Pathology,Yale University School of Medicine, 2Department of Medical Oncology,Yale University School of Medicine

Chapitres

  • 00:04Titre
  • 00:48Bleomycin and Cell Bolus Treatment
  • 03:31Engrafting Lung Adenocarcinoma Cell Lines
  • 05:04Image Analysis to Monitor Tumor Growth
  • 06:33Results: Analysis of Bleomycin Pre-treatment in Athymic Mice
  • 07:58Conclusion

Summary

Traduction automatique

Traduction automatique

We describe a method to significantly enhance orthotopic engraftment of lung cancer cells into the murine lungs by pre-conditioning the airways with injury. This approach may also be applied to study stromal interactions within the lung microenvironment, metastatic dissemination, lung cancer co-morbidities, and to more efficiently generate patient derived xenografts.

Tags

Keywords: Lung CancerTumor-initiating CellsBleomycinOrthotopic EngraftmentPreclinical ModelFibrosisAirway Pre-conditioningCell TransplantationTracheal InjectionMouse Model

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