Journal
/
/
Etablering av en ekstracellulære surt pH kultur-systemet
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Establishment of an Extracellular Acidic pH Culture System
DOI:

09:41 min

November 19, 2017

,

Chapitres

  • 00:05Titre
  • 00:57Preparation of Acidic Culture Medium
  • 02:50Harvesting and Treating Cells
  • 04:31RNA Isolation
  • 06:30cDNA Synthesis and Analysis
  • 08:01Results: Transcriptional Upregulation of Acidic responsive Genes in Response to Lactic Acidosis and HCl-mediated Acidosis
  • 08:49Conclusion

Summary

Traduction automatique

Sure svulst microenvironment spiller avgjørende roller i svulst progresjon. For å vurdere effekten av surt ekstracellulære pH på kreft celler i vitro, etablerte vi enkel surt kultur systemer.

Vidéos Connexes

Read Article