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Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)
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Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)

Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)

DOI:
10.3791/56672-v

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08:13 min

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January 07, 2018

•

,
1Department of Laboratory Medicine,Medical University of Vienna

Chapitres

  • 00:05Titre
  • 00:42Oral Glucose Tolerance Test
  • 04:10Insulin Tolerance test
  • 05:45Results: Glucose Resistance and Compensatory Hyperinsulinemia in Mice Fed a HFD
  • 07:26Conclusion

Summary

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The current article describes the generation and metabolic characterization of high-fat diet-fed mice as a model of diet-induced insulin resistance and obesity. It further features detailed protocols to perform the oral glucose tolerance test and the insulin tolerance test, monitoring whole-body alterations of glucose metabolism in vivo.

Tags

In Vivo Glucose MetabolismHigh-fat DietMiceOral Glucose Tolerance Test (OGTT)Insulin Tolerance Test (ITT)Metabolic PhenotypeGlucose MetabolismGenetic FactorsPharmacological FactorsDietary FactorsBody WeightBlood GlucoseGlucose SolutionGlucose AdministrationGavage

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