/
/
Auditivo de tronco encefálico e exterior cabelo célula célula inteira Patch Clamp gravação em ratos pós-natal
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Department of Physiology, School of Basic Medical Sciences,Southern Medical University, 2School of Basic Medical Sciences,Guizhou Medical University, 3Department of Laboratory Medicines, Guangzhou General Hospital of Guangzhou Military Region,Southern Medical University, 4Experiment Teaching Center, School of Basic Medical Sciences,Southern Medical University
Chapitres
- 00:04Titre
- 00:56Auditory Brainstem Response Recording
- 02:12The Organ of Corti Dissection
- 03:22Immunofluorescence Staining
- 05:21Patch Clamp Recording of Isolated OHCs
- 07:33Results: Whole-Cell Patch Clamp Recording of Isolated Outer Hair Cells
- 08:19Conclusion
Este protocolo descreve métodos para gravar a resposta auditiva do tronco encefálico de filhotes pós-natal. Para examinar o desenvolvimento funcional de células exteriores do cabelo, o procedimento experimental da braçadeira do remendo de células inteiras gravação em células isoladas de cabelo exteriores é descrito passo a passo.
