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Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay
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Journal JoVE Génétique
Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

DOI:
10.3791/56693-v

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11:12 min

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February 22, 2018

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, , , ,
1Department of Biotechnology and Animal Science,National Ilan University, 2Department of Agricultural Biology, College of Agriculture Life Sciences,Chonbuk National University, 3Department of Entomology,National Taiwan University

Chapitres

  • 00:04Titre
  • 00:52Insect Cell Culture
  • 01:53Transient Protein Expression
  • 05:00Anti-apoptotic Activity Assay
  • 07:04Cell Viability Assay
  • 08:11Results: Transient Protein Expression Enables Analysis of Anti-apoptotic Activity
  • 09:41Conclusion

Summary

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This protocol describes a heat shock-induced protein expression system (pDHsp/V5-His/sf9 cell system), which can be used for either expressing foreign proteins or evaluating the anti-apoptotic activity of potential foreign proteins and their truncated amino acids in insect cells.

Tags

Transient Gene ExpressionInsect CellsSf9Protein Functional AssayAnti-apoptotic ActivityHeat Shock InductionProtein ExpressionCell CultureTransfectionIAP3

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