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Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
DOI:

08:55 min

December 29, 2017

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Chapitres

  • 00:05Titre
  • 00:37Preparation of the Microscopy Samples
  • 01:49Recording of the FLIM Data
  • 03:34Conversion of the Photon Recordings to FLIM Images
  • 04:19Fitting of Fluorescence Lifetime Histograms for Whole-cell FLIM Analysis
  • 06:03Results: Syntaxin 4 and VAMP3 Interaction in Live Cell Visualized by FRET-FLIM
  • 07:43Conclusion

Summary

Traduction automatique

This protocol describes a new method allowing for the quantitative visualization of complex formation of SNARE proteins, based on Förster resonance energy transfer, and fluorescence lifetime imaging microscopy.

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