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Immunologie et infection
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En roman mater-fritt System for masseproduksjon av Murine naturlige drepe celler In Vitro
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Immunologie et infection
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Journal JoVE Immunologie et infection
A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

En roman mater-fritt System for masseproduksjon av Murine naturlige drepe celler In Vitro

DOI:
10.3791/56785-v

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10:36 min

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January 09, 2018

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, , , , , , , , ,
1Department of Anatomical and Cellular Pathology,The Chinese University of Hong Kong, 2Li Ka Shing Institute of Health Sciences and Department of Medicine & Therapeutics,The Chinese University of Hong Kong

Chapitres

  • 00:05Titre
  • 01:24Preparation of OP9 Conditional Medium
  • 02:55Isolation of Mouse Bone Marrow Cells
  • 06:14siRNA-mediated Gene Silencing of Differentiating Natural Killer Cells
  • 06:43Analysis of Natural Killer Cell Differentiation Using Flow Cytometry
  • 07:35Results: Study of siRNA Dependent Gene Silencing on the Differentiation of Bone Marrow Derived Natural Killer Cells in a Feeder-free In Vitro System
  • 09:27Conclusion

Summary

Traduction automatique

Traduction automatique

Her presenterer vi en protokoll for å masseprodusere gen-stanse murine NK celler ved hjelp av en mater-fri differensiering system for mekanistisk studie i vitro og i vivo.

Tags

Feeder-free SystemMass ProductionMurine Natural Killer CellsIn VitroInnate ImmunologyNatural Killer Cell DevelopmentCancer ImmunityBone MarrowPersonal ImmunotherapyOP9 CellsAlpha-MEM MediumPhosphate-buffered SalinePentobarbitoneFemur Bones

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