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Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes
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Biologie du développement
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Journal JoVE Biologie du développement
Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes

Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes

DOI:
10.3791/56802-v

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12:46 min

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December 06, 2017

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,
1Department of Biological Sciences,Dartmouth College

Chapitres

  • 00:05Titre
  • 00:50Removal of Chorions and Vitelline Membranes
  • 05:57FISH
  • 10:27Results: Representative Images of Meiotic Figures with Intact Arm Cohesion and Disrupted Arm Cohesion
  • 11:31Conclusion

Summary

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Traduction automatique

This manuscript presents a detailed method for generating X-chromosome arm probes and performing fluorescence in situ hybridization (FISH) to examine the state of sister chromatid cohesion in prometaphase and metaphase I arrested Drosophila oocytes. This protocol is suitable for determining whether meiotic arm cohesion is intact or disrupted in different genotypes.

Tags

Fluorescence In Situ Hybridization (FISH)Drosophila OocytesPrometaphase IMetaphase IMeiotic Arm CohesionMaternal Age EffectOocyte RollingPBSBTXFrosted Glass Slides

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