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Criando um modelo geométrico de elementos finitos estruturalmente realista de um casos para estudar o papel da arquitetura celular em casos de biologia de sistemas
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1Cell Structure and Mechanobiology Group,University of Melbourne, 2Systems Biology Laboratory, Melbourne School of Engineering,University of Melbourne, 3Department of Biomedical Engineering,University of Melbourne, 4School of Mathematics and Statistics, Faculty of Science,University of Melbourne, 5Department of Engineering Science,University of Auckland, 6Advanced Microscopy Facility, Bio21 Molecular Science and Biotechnology Institute,University of Melbourne, 7ARC Centre of Excellence in Convergent Bio-Nano Science and Technology,University of Melbourne, 8School of Medicine, Faculty of Medicine, Dentistry and Health Sciences,University of Melbourne, 9Living Systems Institute,University of Exeter
Chapitres
- 00:04Titre
- 01:19Segment Myofibrils and Mitochondria Regions from the EM 3D Image Dataset
- 03:05Create a Finite Element Mesh from the Segmented Components
- 04:36Mathematically Map the Spatially Varying Density of Ion-channels of Interest onto the Finite Element Mesh
- 06:51Results: A Structurally Realistic Finite Element Geometric Model of a Cardiomyocyte
- 08:13Conclusion
Este protocolo descreve um método novo para criar um modelo de elementos finitos espacialmente detalhada da arquitetura intracelular do cardiomyocytes de imagens de microscopia confocal e microscopia eletrônica. O poder deste modelo espacialmente detalhada é demonstrado usando estudos de caso em sinalização de cálcio e Bioenergética.
