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1Division of Experimental Hematology,Cincinnati Children’s Hospital Medical Center, 2Department of Cell and Developmental Biology, Abramson Family Cancer Research Institute,Perelman School of Medicine, University of Pennsylvania, 3Department of Obstetrics and Gynecology, Southwest Hospital,Third Military Medical University
Chapitres
- 00:04Titre
- 00:48Sample Preparation
- 04:20Electrophoresis, Transfer, and Block
- 05:30Antibody Labeling and Detection
- 06:09Results: Use of Optimized Western Blotting Technique to Analyze Small Numbers of Hematopoietic Stem and Progenitor Cells
- 07:56Conclusion
En standard Western blotting protokollen var optimalisert for å analysere løpet 500 blodkreft stilk eller progenitor celler. Optimalisering innebærer forsiktig håndtering av cellen prøven begrense overføringer mellom rør og direkte lysing cellene i Laemmli eksempel buffer.
