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1Department of Pathology, Immunology and Experimental Medicine,University of Florida, 2Heller School for Social Policy and Management,Brandeis University, 3Department of Medicine, College of Medicine,University of Florida, 4Department of Biomedical Engineering, College of Engineering,University of Florida, 5Department of Pediatrics, College of Medicine,University of Florida
Chapitres
- 00:04Titre
- 00:35Pancreas Fixation and Hydrogel Embedding
- 03:37Tissue Clearing
- 04:38Immunofluorescence Staining
- 07:03Mounting Samples for Imaging
- 08:30Results: Human Neuro-insular Network and 3D Schwann Cell Visualization
- 09:35Conclusion
Vi præsenterer her, en protokol til billede menneskelige bugspytkirtlen sektioner i tre dimensioner (3D) ved hjælp af optimeret passiv clearing metoder. Dette manuskript viser disse procedurer for passiv optisk clearing efterfulgt af flere immunofluorescens farvning for at identificere nøgleelementerne i de autonome og sensoriske neurale netværk innerverer menneskelige Holme.
