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Høyoppløselig 3D-bildebehandling av menneskelig bukspyttkjertelen Nevro-isolerte nettverket
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1Department of Pathology, Immunology and Experimental Medicine,University of Florida, 2Heller School for Social Policy and Management,Brandeis University, 3Department of Medicine, College of Medicine,University of Florida, 4Department of Biomedical Engineering, College of Engineering,University of Florida, 5Department of Pediatrics, College of Medicine,University of Florida
Chapitres
- 00:04Titre
- 00:35Pancreas Fixation and Hydrogel Embedding
- 03:37Tissue Clearing
- 04:38Immunofluorescence Staining
- 07:03Mounting Samples for Imaging
- 08:30Results: Human Neuro-insular Network and 3D Schwann Cell Visualization
- 09:35Conclusion
Her presenterer vi en protokollen bilde menneskelige bukspyttkjertelen delene i tre dimensjoner (3D) med optimalisert passiv fjerne metoder. Dette manuskriptet viser disse prosedyrene for passiv optisk clearing etterfulgt av flere immunofluorescence flekker for å identifisere viktige elementer av autonome og sensoriske nevrale nettverk innervating menneskelige holmer.
