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Immunology and Infection

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JoVE Journal Immunology and Infection
Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion
 

Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion

Article DOI: 10.3791/56866-v • 08:50 min • March 14th, 2018
March 14th, 2018 •
1,2, 3,4,5, 6, 7, 7, 8, 1,2, 3,4,5, 1,2
1Center for Metabolic and Vascular Biology, Mayo Clinic, 2Molecular and Cellular Biosciences, School of Life Sciences, Arizona State University, 3Department of Physics, Arizona State University, 4Center for Biological Physics, Arizona State University, 5Biodesign Institute, Arizona State University, 6Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 7Advanced Imaging Center, HHMI Janelia Research Campus, 8Advanced Light Microscopy Facility, European Molecular Biology Laboratory

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This protocol describes the fabrication of optical-quality glass surfaces adsorbed with compounds containing long-chain hydrocarbons that can be used to monitor macrophage fusion of living specimens and enables super-resolution microscopy of fixed specimens.

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Fabricating Optical-quality Glass Surfaces Study Macrophage Fusion Protocol Transform Non-fusogenic Cover Glass Fusogenic Surface Optical Microscopy Molecular Mechanism Advantage Procedure Microscope Technique Live Imaging James Faust Arnat Balabiyev Well-ventilated Chemical Fume Hood Incubate Hydrochloric Acid Sonication Ultrapure Water Pure Acetone Sterile Ultrapure Water Ethyl Alcohol Long-term Storage Nitrogen Gas Vacuum
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