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Bio-ingénierie
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Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating

Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating

DOI:
10.3791/56879-v

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05:35 min

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June 23, 2018

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, , ,
1Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering,Chinese Academy of Medical Science & Peking Union Medical College

Chapitres

  • 00:04Titre
  • 00:52Fabrication of Heparin-incorporated StarPEG Nanocoating
  • 02:15Mouse Islet Surface Engineering with Heparin-PEG Nanocoating
  • 03:40Results: Characterization of Heparin-PEG Coated Mouse Islets
  • 05:19Conclusion

Summary

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This protocol aims to achieve surface engineering of pancreatic islets using a heparin-incorporated starPEG nanocoating via pseudo-bioorthogonal chemistry between the N-hydroxysuccinimide groups of the nanocoating and the amine groups of islet cell membrane.

Tags

Pancreatic IsletSurface EngineeringHeparinized StarPEG NanocoatingIslet TransplantationCell-based TherapyImmuno-rejectionRevascularizationSurvivalGraft FunctionTherapeutic EfficacyCell RetentionCell SurvivalHeparinNHSEDCStarPEGMouse IsletsPBSRPMI 1640FBS

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