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Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac
Journal JoVE
Environnement
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Journal JoVE Environnement
Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac
DOI:

07:57 min

March 15, 2018

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Chapitres

  • 00:05Titre
  • 00:51Growth Conditions to Induce Adventitious Shoots of Ipecac
  • 01:46Extraction and Purification of Phytohormones
  • 04:46LC-MS/MS Analysis of IAA and CKs
  • 05:41Results: IAA and CK Levels in Adventitious Shoots Change During Five Weeks of Culture
  • 07:12Conclusion

Summary

Traduction automatique

Adventitious shoots can be induced on internodal segments of ipecac without phytohormone treatment. To evaluate phytohormone dynamics during adventitious shoot formation, we measured endogenous auxin and cytokinin in internodal segments by LC-MS/MS.

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