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无洗涤剂超快重组膜蛋白成脂双层使用融合补充充电 Proteoliposomes。
Journal JoVE
Biochimie
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Journal JoVE Biochimie
Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
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无洗涤剂超快重组膜蛋白成脂双层使用融合补充充电 Proteoliposomes。

DOI:
10.3791/56909-v

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11:10 min

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April 05, 2018

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, , , ,
1First Pavlov State Medical University, 2Clarendon Laboratory, Department of Physics,Oxford University, 3Department of Biological Sciences,Southern Methodist University

Chapitres

  • 00:04Titre
  • 01:16Formation of Fusogenic GUV by Inverted Emulsion Method
  • 05:59Delivery of Membrane Proteins and Assembly of Electron Transport Chain in the LUV and GUV followed by ATP Production
  • 08:21Results: Use of Vesicle Fusion and ACMA Quenching Assay to Analyze the Assembly of Complex Multi-component Membrane Systems
  • 10:11Conclusion

Summary

Traduction automatique

Traduction automatique

在这里, 我们提出了两个超快的协议, 以重组膜蛋白到融合 proteoliposomes, 并融合这样的 proteoliposomes 与靶脂双层, 以无洗涤剂提供这些膜蛋白到 postfusion 双层。这些方法的结合使复杂的多组分膜系统能够快速、容易地控制装配。

Tags

Membrane ProteinsLipid BilayersFusogenic ProteoliposomesDetergent-freeReconstitutionArtificial CellMembrane TransportSynthetic Biology

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