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Genome-wide RNAi Screening at identificere vært faktorer, at modulerer Oncolytic Virus terapi
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1Children’s Hospital of Eastern Ontario (CHEO) Research Institute, 2Department of Biology, Microbiology and Immunology,University of Ottawa, 3Department of Pediatrics,University of Ottawa, 4Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine,University of Calgary
Chapitres
- 00:05Titre
- 00:45Preparation for Reverse Transcription
- 01:57Reverse Transfection
- 04:32Infection
- 05:52Fixing and Staining Cells
- 06:39Results: Genome-wide RNAi Screen to Identify Host Targets that Enhance Oncolytic Virus Therapy
- 08:13Conclusion
Her beskriver vi en protokol for at ansætte høj overførselshastighed RNAi screening at afdække vært mål, der kan manipuleres til at forbedre oncolytic virus terapi, specielt rhabodvirus og vaccinia virus behandling, men det kan være let tilpasses til andre oncolytic virus platforme eller for at opdage vært gener, at modulere virus replikering generelt.
