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Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity
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Chapitres
- 00:04Titre
- 01:06Culture Preparation of Dissociated Adult DRG Neuron
- 03:41Co-culture of Primary Peritoneal Macrophages on A Cell Culture Insert
- 05:47Neurite Outgrowth Assay with Collected Conditioned Medium
- 07:05Results: Representative Results of Neurite Outgrowth Assay Using Conditioned Medium Obtained from Various Conditions
- 07:55Conclusion
The current protocol presents experimental procedures to stimulate cultured macrophages to be endowed with capacity to release molecular factors that promote neurite outgrowth. Treatment of cAMP to the neuron-macrophage co-cultures induces the macrophages to produce conditioned medium that possesses strong neurite outgrowth activity.
