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Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
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Journal JoVE Recherche en cancérologie
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

DOI:
10.3791/56976-v

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09:01 min

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March 26, 2018

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, , , , , , , , ,
1Laboratoire d’hématologie,CHU Lille, 2Univ. Lille, Inserm, CHU Lille, UMR-S 1172 – JPArc – Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer, 3Service d’hématologie pédiatrique, hôpital Jeanne de Flandre,CHU Lille, 4Laboratoire d’hématologie CH Lyon Sud, 5Service des Maladies du Sang, hôpital Claude Huriez,CHU Lille

Chapitres

  • 00:04Titre
  • 00:58Sample Preparation and Lysis of Red Blood Cells
  • 02:26Patient Derived Xenografts and Staining
  • 04:50Flow Cytometry and Gating Strategy
  • 06:14Results: Parametric Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia
  • 07:57Conclusion

Summary

Traduction automatique

Traduction automatique

We outline a protocol to detect simultaneous expression of leukemia stem cell markers on primary acute myeloid leukemia cells by flow cytometry. We show how to quantify three progenitor populations and a putative LSC population with increasing degree of maturation. We confirmed the presence of these populations in corresponding patient-derived-xenografts.

Tags

Keywords: Flow CytometryLeukemia Stem CellsAcute Myeloid LeukemiaPatient-derived XenograftsDiagnosisFollow-upMononuclear CellsDensity GradientRed Blood Cell LysisNSG MouseXenograftSubmandibular Blood Collection

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