Journal
/
Immunologie et infection
/
A Recovery Cardiopulmonary Bypass Model Without Transfusion or Inotropic Agents in Rats
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
A Recovery Cardiopulmonary Bypass Model Without Transfusion or Inotropic Agents in Rats

A Recovery Cardiopulmonary Bypass Model Without Transfusion or Inotropic Agents in Rats

DOI:
10.3791/56986-v

•

09:54 min

•

March 23, 2018

•

, , ,
1Department of Cardiovascular Surgery, Graduate School of Medicine,Kyoto University

Chapitres

  • 00:05Titre
  • 00:41Preparing the CardioPulmonary Bypass (CPB) Circuit
  • 01:56Preparing the Animal for CPB
  • 03:28Cannulation
  • 05:12The CPB Procedure
  • 08:01Results: Physiological Changes with CPB and Histological Analysis
  • 09:22Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a protocol to describe a simple recovery cardiopulmonary bypass model without transfusion or inotropic agents in a rat. This model allows the study of the long-term multiple organ sequelae of cardiopulmonary bypass.

Tags

Keywords: Cardiopulmonary BypassCPBRecovery ModelTransfusion-freeInotropic Agent-freeRodent ModelOrgan ComplicationsCardiovascular Cellular FeedCardiopulmonary Bypass SurgeryPriming SolutionMembrane OxygenatorAnesthesiaTemperature MaintenanceECG MonitoringCannulationFemoral ArteryJugular Vein

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/56986?article_type=v

Diagnosis of Ecto- and Endoparasites in Laboratory Rats and Mice
check_url/fr/56986?article_type=v

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus
check_url/fr/56986?article_type=v

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
check_url/fr/56986?article_type=v

A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii
check_url/fr/56986?article_type=v

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
check_url/fr/56986?article_type=v

A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia
check_url/fr/56986?article_type=v

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
check_url/fr/56986?article_type=v

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry
check_url/fr/56986?article_type=v

Visualization and Quantification of High-Dimensional Cytometry Data using Cytofast and the Upstream Clustering Methods FlowSOM and Cytosplore
check_url/fr/56986?article_type=v

An Immunological Model for Heterotopic Heart and Cardiac Muscle Cell Transplantation in Rats

Read Article