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来自沙柯玛患者衍生异种移植物的肿瘤启动细胞的分离和表征
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
DOI:

07:18 min

June 13, 2019

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Chapitres

  • 00:04Titre
  • 00:44Sarcoma Tissue Sample Processing and Patient-derived Xenograft (PDX) Formation
  • 02:32Tumor-initiating Cell (TIC) Sorting and Sarcosphere Formation
  • 05:11Results: Representative Human Leukocyte Antigen (HLA)-1-Negative TIC Characterization
  • 06:42Conclusion

Summary

Traduction automatique

我们描述了通过荧光活性细胞分类,使用人类白细胞抗原-1(HLA-1)作为阴性标记,从人类肉瘤患者衍生的异种移植物分离肿瘤启动细胞的详细方案,以及进一步的验证和这些HLA-1阴性肿瘤启动细胞的表征。

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