Journal
/
Médecine
/
An Optimized Evans Blue Protocol to Assess Vascular Leak in the Mouse
Journal JoVE
Médecine
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Médecine
An Optimized Evans Blue Protocol to Assess Vascular Leak in the Mouse

An Optimized Evans Blue Protocol to Assess Vascular Leak in the Mouse

DOI:
10.3791/57037-v

•

06:39 min

•

September 12, 2018

•

, , ,
1Cardiovascular Pulmonary Research Laboratory,University of Colorado Denver, 2Division of Pulmonary Sciences and Critical Care Medicine,University of Colorado Denver, 3Denver VA Medical Center

Chapitres

  • 00:04Titre
  • 00:59Catheter Insertion
  • 03:12Injections and Organ Isolation
  • 04:50Results: Representative Substance P-induced Extravasation Analyses
  • 05:49Conclusion

Summary

Traduction automatique

Traduction automatique

In this article, an economical, optimized, and simple protocol is described which uses the Evans blue dye method for assessing plasma extravasation in the organs of FVBN mice that can be adapted for use in other strains, species, and other organs or tissues.

Tags

Keywords: Evans Blue ProtocolVascular LeakPlasma ExtravasationMouseCatheterizationJugular VeinEvans Blue DyeVascular FieldOrgan Of InterestSurgical TechniqueHeparinized Saline

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/57037?article_type=v

Technical Aspects of the Mouse Aortocaval Fistula
check_url/fr/57037?article_type=v

Diagnostic Ultrasound Imaging of Mouse Diaphragm Function
check_url/fr/57037?article_type=v

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis
check_url/fr/57037?article_type=v

Using an Ingestible Telemetric Temperature Pill to Assess Gastrointestinal Temperature During Exercise
check_url/fr/57037?article_type=v

Intraluminal Drug Delivery to the Mouse Arteriovenous Fistula Endothelium
check_url/fr/57037?article_type=v

Using the Sleeve Technique in a Mouse Model of Aortic Transplantation – An Instructional Video
check_url/fr/57037?article_type=v

Intravital Microscopy and Thrombus Induction in the Earlobe of a Hairless Mouse
check_url/fr/57037?article_type=v

An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers
check_url/fr/57037?article_type=v

Optimized Griess Reaction for UV-Vis and Naked-eye Determination of Anti-malarial Primaquine
check_url/fr/57037?article_type=v

An In Vitro Hemodynamic Loop Model to Investigate the Hemocytocompatibility and Host Cell Activation of Vascular Medical Devices

Read Article