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使用河豚毒素耐药性钠通道检查果蝇中的 Monosynaptic 连接
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Journal JoVE Neurosciences
Examining Monosynaptic Connections in Drosophila Using Tetrodotoxin Resistant Sodium Channels
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使用河豚毒素耐药性钠通道检查果蝇中的 Monosynaptic 连接

DOI:
10.3791/57052-v

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09:55 min

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February 14, 2018

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,
1Department of Biology,University of Maryland

Chapitres

  • 00:04Titre
  • 00:42Preparation
  • 03:17Obtaining a Whole-cell Recording Configuration
  • 05:36Testing for Synaptic Connectivity
  • 07:17Results: Using Tetrotoxin Engineered Resistance for Probing Synapses (TERPS)
  • 08:58Conclusion

Summary

Traduction automatique

Traduction automatique

本文的特点是通过使用河豚毒素和河豚毒素耐药钠通道, NaChBac 来检测神经元之间的 monosynaptic 连接。

Tags

Monosynaptic ConnectionsDrosophilaTetrodotoxin Resistant Sodium ChannelsElectrophysiologyDissectionPatch-clamp RecordingsRecording SalineCsChrimsonAll-trans-RetinalAntennal LobesGlial Sheathing

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