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1Laboratory of Translational Immunology,University Medical Center Utrecht, Utrecht University, 2Department of Rheumatology & Clinical Immunology,University Medical Center Utrecht, Utrecht University, 3Laboratory of Neuroimmunology and Developmental Origins of Disease,University Medical Center Utrecht, Utrecht University
Chapitres
- 00:05Titre
- 01:15Cell Transfection, Protein Purification, and Affinity Chromatrography
- 03:49Bioactivity of IL4-10 Fusion Protein
- 04:46Induction of Chronic Inflammatory Pain in Mice. Pain Measurements: The Von Frey Test: Mechanical Sensitivity
- 05:52Pain measurements: Hargreaves Test for Thermal Sensitivity Dynamic Weight Bearing Test for Postural Deficits
- 06:48Intrathecal Injection of IL4-10 Fusion Protein for Analgesia
- 07:56Results: Persistent Inflammatory Pain Reduction Using IL4-10 Fusion Protein
- 09:38Conclusion
In questo documento vengono forniti dettagli per i metodi di produzione e controllo di qualità di una proteina di fusione ricombinante IL4-10. Mostriamo anche come testare l'efficacia di questa proteina a risolvere il dolore in un modello murino di dolore infiammatorio.
