Here we present a protocol for cardiac-specific gene manipulation in mice. Under anesthesia, the mouse hearts were externalized through the fourth intercostal space. Subsequently, adenoviruses encoding specific genes were injected with a syringe into the myocardium, followed by protein expression measurement via in vivo imaging and Western blot analysis.

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Pyrosequencing: A Simple Method for Accurate Genotyping

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions

In Vivo SiRNA Transfection and Gene Knockdown in Spinal Cord via Rapid Noninvasive Lumbar Intrathecal Injections in Mice

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector

A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice

•

•

•

Chapitres

Summary

Traduction automatique

Tags