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Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
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Journal JoVE Biologie
Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

DOI:
10.3791/57087-v

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14:23 min

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March 06, 2018

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, , ,
1Cell Polarity, Migration and Cancer Unit, UMR 3691, CNRS,Institut Pasteur, 2UTechS Photonic BioImaging (Imagopole) Citech,Institut Pasteur

Chapitres

  • 00:04Titre
  • 00:54Cell Fixation
  • 02:25Preparing Samples and Image Acquisition
  • 10:10Image Reconstruction
  • 11:50Results: 2-dimensional Direct Stochastic Optical Reconstruction Microscopy of Intermediate Filaments
  • 12:54Conclusion

Summary

Traduction automatique

Traduction automatique

The overall goal of this methodology is to give the optimal experimental conditions from sample preparation to image acquisition and reconstruction in order to perform 2D dual color dSTORM images of microtubules and intermediate filaments in fixed cells

Tags

Keywords: 2D D-STORMIntermediate FilamentsMicrotubulesCytoskeletal NetworksSample PreparationImmunostainingFluorescence MicroscopyImaging BufferAcquisition Parameters

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