Journal
/
Biologie
/
Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds

DOI:
10.3791/57127-v

•

08:54 min

•

February 14, 2018

•

, , , , , ,
1Laboratory of Molecular Biology, Department of Cellular Biology, Institute of Biological Sciences,University of Brasília, 2Department of Microbiology and Immunology,Albert Einstein College of Medicine, 3Division of Infectious Diseases, Department of Medicine,Albert Einstein College of Medicine, 4Faculty of Ceilândia,University of Brasília

Chapitres

  • 00:04Titre
  • 00:47Preparing Fungal Inoculum and Peptides
  • 02:48Antifungal Assay
  • 05:23Results: Detection of New Antimicrobial Peptides
  • 07:10Conclusion

Summary

Traduction automatique

Traduction automatique

An easy and adaptable broth microdilution method for screening antifungal compounds and extracts.

Tags

Keywords: Broth MicrodilutionIn Vitro ScreeningAntifungal CompoundsFungal ActivityRPMI 1640 MediumC. AlbicansC. NeoformansPeptidesSerial Dilution96-well Microplate

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/57127?article_type=v

Using Laser Tweezers For Manipulating Isolated Neurons In Vitro
check_url/fr/57127?article_type=v

Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro
check_url/fr/57127?article_type=v

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
check_url/fr/57127?article_type=v

Construction of an Affordable and Easy-to-Build Zebrafish Facility
check_url/fr/57127?article_type=v

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants
check_url/fr/57127?article_type=v

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
check_url/fr/57127?article_type=v

An Easy Method for Plant Polysome Profiling
check_url/fr/57127?article_type=v

Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products
check_url/fr/57127?article_type=v

An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector
check_url/fr/57127?article_type=v

An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants

Read Article