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A Burrowing/Tunneling Assay for Detection of Hypoxia in Drosophila melanogaster Larvae
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Chapitres
- 00:04Titre
- 01:03Preparation of the Larvae and Assay Plates
- 02:52Conducting the Assay
- 04:24Preparing Assay Plates for Imaging
- 07:06Results: Assessing RNAi Suppression of uninflatable
- 08:32Conclusion
The protocol describes a simple assay to identify Drosophila melanogaster larvae that are experiencing hypoxia under normal atmospheric oxygen levels. This protocol allows hypoxic larvae to be distinguished from other mutants that show overlapping phenotypes such as sluggishness or slow growth.
