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1School of Biological Sciences,The University of Auckland, 2Research School of Biology,The Australian National University, 3Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences,The University of Auckland, 4Department of Plastic Surgery,Counties Manukau District Health Board, 5Department of Surgery, Faculty of Medical and Health Sciences,The University of Auckland, 6Biomedical Imaging Research Unit, Faculty of Medical and Health Sciences,The University of Auckland, 7Maurice Wilkins Centre,The University of Auckland
Chapitres
- 00:00Titre
- 01:55Inducing the differentiation of human adipose derived stromal cells
- 03:36Staining for differentiation
- 05:53Imaging cells using high content screening microscope
- 08:23Image Analysis: Cell Scoring
- 09:43Image Analysis: Transfluor
- 10:43Representative results
Los métodos tradicionales de evaluación de diferenciación adipogenic son baratos y fácil de usar, pero no son específicos a los cambios en la expresión génica. Hemos desarrollado un ensayo para cuantificar la diferenciación de células mesenquimales en adipocytes maduros usando un marcador específico de linaje. Este ensayo tiene diversas aplicaciones en investigación básica y la medicina clínica.
