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实验性曲霉病肺真菌负担的组织学定量测定
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
DOI:

09:52 min

March 09, 2018

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Chapitres

  • 00:05Titre
  • 01:04Aspergillus Fumigatus Conidia Infection
  • 03:09Mouse Lung Harvest and Preservation
  • 05:09Light Microscopy Imaging and Fungal Burden Calculation
  • 07:26Results: Representative Survival, Fungal Burden, and Fungal Clearance in a Mouse Model of Invasive Aspergillus Infection
  • 08:53Conclusion

Summary

Traduction automatique

在这里我们描述了一个协议, 以确定侵袭性曲霉病小鼠肺部真菌负担的定量戈木日修饰甲胺银染色的组织学切片。这种方法的应用结果与较少的动物相比, 通过定量 PCR 的肺真菌 DNA 评估的真菌负担比较。

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