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Histologiske kvantificering at bestemme lunge svampe byrde i eksperimentel aspergillose
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
DOI:

09:52 min

March 09, 2018

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Chapitres

  • 00:05Titre
  • 01:04Aspergillus Fumigatus Conidia Infection
  • 03:09Mouse Lung Harvest and Preservation
  • 05:09Light Microscopy Imaging and Fungal Burden Calculation
  • 07:26Results: Representative Survival, Fungal Burden, and Fungal Clearance in a Mouse Model of Invasive Aspergillus Infection
  • 08:53Conclusion

Summary

Traduction automatique

Her beskriver vi en protokol for at fastslå pulmonal svampe byrde i mus med invasiv aspergillose ved kvantificeringen af Gomoris modificerede methanamine sølvfarvning i histologiske sektioner. Brug af denne metode resulterede i sammenlignelige resultater med mindre dyr i forhold til vurdering af svampe byrde af kvantitativ PCR lunge svampe DNA.

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