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A Method for Islet Transplantation to the Omentum in Mouse
 

A Method for Islet Transplantation to the Omentum in Mouse

Article DOI: 10.3791/57160-v • 06:25 min • January 7th, 2019
January 7th, 2019 •
*1, *1, *2, 2, 3, 1, 1, 1, 1, 1, 1, 4, 5, 1, 1
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, Shenzhen University Health Science Center, 2Institute for Cellular Therapeutics, Allegheny-Singer Research Institute, 3Xenotransplantation Program/Department of Surgery, The University of Alabama at Birmingham, 4College of Life Science and Oceanography, Shenzhen University, 5School of Pharmcy, Wenzhou Medical College
* These authors contributed equally

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résumé
Automatic Translation

A method for the omental transplantation of islets in a mouse is introduced. The isolated islets are mixed with hydrogel and the mixture is placed into the omental pouch of a diabetic mouse. Then, the blood glucose is monitored, and immuno-histochemical analysis is performed.

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Keywords: Islet Transplantation Omentum Mouse Type I Diabetes Hydrogel Anesthesia Surgery Sterile Technique Centrifugation Pipette
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