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Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

DOI:
10.3791/57166-v

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06:47 min

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May 04, 2018

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, , ,
1Chang Gung University, 2Department of Periodontics,Chang Gung Memorial Hospital, 3California Northstate University College of Medicine, 4Department of Nephrology, Clinical Poison Center,Chang Gung Memorial Hospital, 5Kidney Research Center,Chang Gung Memorial Hospital, 6Center for Tissue Engineering,Chang Gung Memorial Hospital, 7Department of Periodontics,Chang Gung Memorial Hospital, 8College of Oral Medicine,Taipei Medical University

Chapitres

  • 00:05Titre
  • 01:10Tissue Preparations
  • 03:15Cell Attachment and Viability with Osteogenic Differentiation
  • 04:28Results: Osteogenic Differentiation of PDCs and Analysis of 1,25-dihydroxy Vitamin D (Calcitriol) and Vitamin C Treatment of PDCs using RT-PCR
  • 06:11Conclusion

Summary

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Traduction automatique

We present a protocol to investigate the mRNA expression biomarkers of periosteum-derived cells (PDCs) induced by vitamin C (vitamin C) and 1,25-dihydroxy vitamin D [1,25-(OH)2D3]. In addition, we evaluate the ability of PDCs to differentiate into osteocytes, chondrocytes, and adipocytes.

Tags

Mesenchymal Stem CellsAlveolar PeriosteumVitamin DOsteogenic ActivityCell IsolationCell CultureBone FormationOsteoinductive Potential

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