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Immunologie et infection

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脂多糖治疗小鼠肝脏免疫应答中白细胞的双光子活体成像

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Immunologie et infection

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Journal JoVE Immunologie et infection

Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver

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脂多糖治疗小鼠肝脏免疫应答中白细胞的双光子活体成像

DOI:

10.3791/57191-v

•

07:06 min

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February 06, 2018

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Sang A Park*^1,2, Young Ho Choe*^1,2, Sung Hwan Lee, Young-Min Hyun²

¹Department of Anatomy,Yonsei University College of Medicine, ²Brain Korea 21 Plus Project for Medical Science,Yonsei University College of Medicine, ³Department of Surgery,Yonsei University College of Medicine

Chapitres

00:05Titre
00:28Surgical Process
03:06Intravital Imaging of the Liver with Two-photon MIcroscopy
04:51Results: Two-photon Intravital Imaging in LPS-treated Mouse Liver
06:03Conclusion

Summary

Traduction automatique

我们建立了一种新的小鼠肝脏双光子成像的手术方案。利用这一技术, 我们确定了肝内毒素诱导中的内毒素血症的详细结构。我们预期这种方法可以用来确定各种试剂治疗肝白细胞迁移的有效性。

Tags

Keywords: Two-photon Intravital Imaging Leukocytes Immune Response Lipopolysaccharide Mouse Liver Septic Shock Liver Surgery Anesthesia Texas Red Dextran Povidone Iodine Abdominal Cavity Liver Exposure Intravital Imaging Laser Settings Water Immersion Objective Lens Temperature Control

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