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Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model
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Médecine
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Journal JoVE Médecine
Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model

Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model

DOI:
10.3791/57213-v

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09:58 min

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August 11, 2018

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, , , , , , ,
1Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine,Utrecht University, 2Department of Otorhinolaryngology,Erasmus MC University Medical Center, 3Department of Clinical Sciences and Public Health, Faculty of Veterinary Science,Mahidol University, 4Department of Surgery,Erasmus MC University Medical Center, 5Department of Orthopaedics,Erasmus MC University Medical Center, 6Hill’s Pet Nutrition Inc.

Chapitres

  • 00:04Titre
  • 01:14Human Adipose Stem Cell (ASC) Isolation
  • 04:01ASC Subculture
  • 05:16ASC Sheet Preparation
  • 07:08ASC Sheet Transplantation
  • 08:24Results: Colorectal Anastomosis Macroscopic and Histological Evaluation
  • 09:17Conclusion

Summary

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The preparation and transplantation of adipose tissue derived stem cell (ASC) sheets onto insufficiently sutured colorectal anastomoses in a rat model is presented. This novel application shows that ASC sheets can reduce colorectal anastomosis leakage.

Tags

ASC SheetsPartial ColectomyIntestinal AnastomosisLeakage PreventionIntra-abdominal OrgansCell IsolationCell CultureCell CountingCell Seeding

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