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复杂核型细胞周期捕获细胞的生成与分离
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes
DOI:

05:22 min

April 13, 2018

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Chapitres

  • 00:04Titre
  • 00:32Synchronization of RPE-1 hTERT Cells
  • 01:34Generation of Aneuploid Cells by Interference with the Activity of the Mitotic Kinase Mps1 and Removal of Aneuploid Cycling Cells to Enrich the ArCK Population
  • 03:36Results: In Vitro Isolation and Characterization of Aneuploid Cells with Complex Karyotypes (ArCK)
  • 04:35Conclusion

Summary

Traduction automatique

体检导致基因组不稳定, 最终产生细胞周期-被捕获的细胞与复杂的核型。本文提供了一种简单方便的分离异倍体细胞的方法。

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