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1Stem Cells & Regenerative Medicine Center,Baylor College of Medicine, 2Center for Cell and Gene Therapy,Baylor College of Medicine, 3Centro di Ricerca Emato-Oncologica (CREO),University of Perugia, 4Department of Molecular & Human Genetics,Baylor College of Medicine, 5Texas Children’s Hospital & Houston Methodist Hospital
Chapitres
- 00:05Titre
- 01:05sgRNA Fwd Primer Design, DNA Template Synthesis, and ln Vitro Transcription of sgRNA
- 03:38Cas9-sgRNA Complexing and Electroporation
- 05:45Results: Use of Cas9-sgRNA Electroporation Protocol to Study Efficient Knockout of Target Gene
- 07:48Conclusion
En protokoll for rask CRISPR/Cas9-mediert genet avbrudd i musen og menneskelige primære blodkreft cellene er beskrevet i denne artikkelen. Cas9-sgRNA ribonucleoproteins blir introdusert via electroporation sgRNAs generert gjennom i vitro transkripsjon og kommersielle Cas9. Høy redigering effektivitet oppnås med begrenset tid og finansielle kostnader.
