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Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

DOI:
10.3791/57314-v

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11:37 min

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February 03, 2018

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, , , , , , , , , , ,
1Department of Chemical and Materials Engineering,National Central University, 2Department of Botany and Microbiology,King Saud University, 3Cathay Medical Research Institute,Cathay General Hospital, 4Graduate Institute of Systems Biology and Bioinformatics,National Central University, 5Department of Medical Microbiology and Parasitology,Universiti Putra Malaysia, 6Department of Internal Medicine,Taiwan Landseed Hospital, 7Department of Zoology,Bharathiar University, 8Thiruvalluvar University

Chapitres

  • 00:04Titre
  • 01:00Polyvinyl Alcohol-Co-Itaconic Acid (P-IA) Hydrogel Dish Preparation and Grafting
  • 05:02Passaging of Undifferentiated Human Embryonic Stem (ES) and Human Induced Pluripotent Stem (iPS) Cells on Hydrogels
  • 06:37Characterization of the Expression of Pluripotency Proteins by Immunostaining
  • 08:49Results: Human ES and iPS Pluripotency on Conventional Dishes and Hydrogel Dishes
  • 10:19Conclusion

Summary

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Traduction automatique

A protocol is presented to prepare polyvinyl alcohol-co-itaconic acid hydrogels with varying stiffness, which were grafted with and without oligopeptides, to investigate the effect of the stiffness of biomaterials on the differentiation and proliferation of stem cells. The stiffness of the hydrogels was controlled by the crosslinking time.

Tags

Keywords: Polyvinyl Alcohol-co-itaconic Acid HydrogelsStem Cell CultureXeno-free ConditionsHydrogel ElasticityStem Cell DifferentiationCrosslinkingHydrogel PreparationP-IA Films

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