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rna 干扰的实际应用: 口服双链 rna 在脂质体载体为蟑螂

Journal JoVE
Biologie

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Journal JoVE Biologie

Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches

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rna 干扰的实际应用: 口服双链 rna 在脂质体载体为蟑螂

DOI:

10.3791/57385-v

•

08:26 min

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May 01, 2018

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Jia-Hsin Huang, Yun Liu, Yu-Hsien Lin^2,3, Xavier Belles, How-Jing Lee

¹Department of Entomology,National Taiwan University, ²Biology Centre, Institute of Entomology,Czech Academy of Sciences, ³Faculty of Science,University of South Bohemia, ⁴Institute of Evolutionary Biology, CSIC-UPF

Chapitres

00:04Titre
00:50Collection of Extracellular Enzymes from Hemolymph
02:13Collection of Extracellular Enzymes from Midgut Juice
03:31dsRNA Degradation Assay
05:31Oral Administration of dsRNA
06:42Results: Knock Down of Tubulin Expression in the Midgut
07:38Conclusion

Summary

Traduction automatique

本手稿通过口服双链 RNA 包裹在脂质体中, 证明了德国蟑螂中肠内基因表达的耗竭。

Tags

RNA Interference Double-stranded RNA Liposome Carriers Cockroaches Pest Control Gene Silencing Hemolymph Collection Midgut Juice Insect Saline Buffer Protein Quantification

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