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Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

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Biologie du développement

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Journal JoVE Biologie du développement

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

DOI:

10.3791/57414-v

•

06:18 min

•

April 13, 2018

•

Kunal Baxi, Carlos E. de Carvalho

¹Department of Biology,University of Saskatchewan

Chapitres

00:04Titre
01:01Staining and Imaging of Intestinal Lysosomes
04:24Results: Study of the Lysosomal Functional Activity Using a pH Sensitive Dye
05:38Conclusion

Summary

Traduction automatique

A step-by-step guide to probe loss of lysosomal acidity in the intestine of C. elegans using the pH-sensitive vital dye 5(6)-carboxy-2',7'-dichlorofluorescein diacetate (cDCFDA)

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