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Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model
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Journal JoVE Biologie
Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model

Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model

DOI:
10.3791/57466-v

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10:20 min

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August 10, 2018

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, , , , , ,
1Postgraduate institution,Guangzhou Medical University, 2Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, 3Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopaedic Engineering,Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), 4Department of Chemistry,Chinese University of Hong Kong, 5Shenzhen Kangning Hospital,Shenzhen Mental Health Center

Chapitres

  • 00:04Titre
  • 01:07Isolate and Culture the rbSF-MSCs
  • 03:21CD90-positive Magnetic Activated Cell Sorting (MACS) of the rbSF-MSCs and Primary Culture
  • 05:01Magnetic Separation and Culture of te CD90+ rbSF-MSCs
  • 06:44Results: Obtain Purified Rabbit SF-MSCs by CD90-Based Magnetic Activated Cell Sorting
  • 08:39Conclusion

Summary

Traduction automatique

Traduction automatique

This article presents a simple and economic protocol for the straightforward isolation and purification of mesenchymal stem cells from New Zealand white rabbit synovial fluid.

Tags

Magnetic-activated Cell SortingMesenchymal Stem CellsSynovial FluidRabbit ModelIsolationPurificationFDA-approvedCell CultureCell SuspensionCentrifugationCell StrainerCell ColoniesCell Passaging

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