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Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections
Journal JoVE
Biologie
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Journal JoVE Biologie
Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections

Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections

DOI:
10.3791/57475-v

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06:26 min

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March 21, 2018

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, , , , , , ,
1Division of Perinatology, Institute for Developmental Research,Aichi Human Service Center, 2Surgery Department,Anjo Kosei Hospital, 3Department of Pathology,Nagoya University Graduate School of Medicine

Chapitres

  • 00:04Titre
  • 00:40Dissection and Isolation of Jejunum
  • 02:40Immunofluorescence of Tuft Cells Using Free-floating Cryosections
  • 04:42Results: Representative Fluorescence Images of Mouse Intestinal Tuft Cells
  • 05:55Conclusion

Summary

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Kuga et al. discovered that phosphorylation-status specific antibodies against the actin binding protein girdin phosphorylated at tyrosine 1798 (pY1798) can be used to label tuft cells (TCs). This protocol allows robust visualization of TCs using immunofluorescent staining of free-floating jejunum cryosections with pY1798 antibodies.

Tags

Keywords: Anti-phospho-girdin AntibodiesTuft CellsMouse JejunumCryosectionsParasite InfectionGastroenterologyHistological AnalysisIntestinal ContentsFormalin FixationGelatin EmbeddingCryosectioning

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