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Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study
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Médecine
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Journal JoVE Médecine
Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study

Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study

DOI:
10.3791/57492-v

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08:00 min

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April 11, 2018

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, , , , , ,
1Department of Pediatrics, Division of Pulmonary Medicine,Cincinnati Children’s Hospital Medical Center

Chapitres

  • 00:04Titre
  • 00:41Passage Human Nasal Epithelial Cells
  • 02:45Seeding Cells for HNE Spheroid Cultures
  • 04:37Culture Differentiated and Mature HNE Spheroids
  • 05:27Results: Individualized CFTR Study Through the Generation of HNE Spheroids
  • 07:01Conclusion

Summary

Traduction automatique

Traduction automatique

Here we describe a method to generate three-dimensional spheroid cultures of human nasal epithelial cells. Spheroids are then stimulated to drive Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)-dependent ion and fluid secretion, quantifying the change in the spheroid luminal size as a proxy for CFTR function.

Tags

Nasal Epithelial Cell SpheroidsCFTR StudiesCystic FibrosisEx Vivo ModelCell CultureTrypsinizationCell ExpansionCell DifferentiationBasement Membrane Matrix

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