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An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

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Génétique

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Journal JoVE Génétique

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

DOI:

10.3791/57494-v

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06:21 min

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May 07, 2018

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Seoghyun Lee*¹, Minho Won*², Ran Hee Hwang, Gang Min Hur, Hyunju Ro

¹Department of Biological Sciences, College of Bioscience and Biotechnology,Chungnam National University, ²Department of Pharmacology, College of Medicine,Chungnam National University, ³Department of Nursing,Gwangju Women’s University

Chapitres

00:05Titre
00:45Transient Transfection and Teb Treatment
03:00Depletion of Teb
04:01Results: Modulation of Transgene Expression of the pEUI() Singular Gene Switch using Teb
05:11Conclusion

Summary

Traduction automatique

Here, we present a protocol for the modulation of transgene expression using pEUI(+) singular gene switch by tebufenozide treatment.

Tags

Ecdysone Receptor Gene Switch Transgene Expression Tebufenozide HEK 293 Cells EGFP ANKRD13A Transfection Immunoblotting

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